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The second response cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as items (Fig. 9) - spectrophotometers. As drawn in Fig - https://padlet.com/julieanndesalorenz30606/olis-clarity-srqqvp7768okh664. 9, the response may also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As revealed in Fig. 9, this might be because the H4B radical function as an electron sink in a later point in the reaction, so regarding allow NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.